Blood-respiratory and acid-base changes during extended diving in the bimodally respiring freshwater turtle Rheodytes leukops

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Vol. 174; no. 4; pp. 347 - 354
• Main Authors: Gordos, Matthew A, Franklin, Craig E, Limpus, Colin J, Wilson, Gary
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01-05-2004
• Subjects: Acid-Base Equilibrium - physiology; Animals; Blood; Blood Chemical Analysis; Blood Gas Analysis; Diving; Fresh Water; Freshwater; Oxygen - blood; Physical Exertion - physiology; Queensland; Respiration; Rheodytes leukops; Time Factors; Turtles - physiology; Queensland
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-004-0420-x

Changes in blood-gas, acid-base, and plasma-ion status were investigated in the bimodally respiring turtle, Rheodytes leukops, during prolonged dives of up to 12 h. Given that R. leukops routinely submerges for several hours, the objective of this study was to determine whether voluntarily diving turtles remain aerobic and simultaneously avoid hypercapnic conditions over increasing dive lengths. Blood PO(2), PCO(2), and pH, as well as plasma concentrations of lactate, glucose, Na(+), K(+), Cl(-), total Ca, and total Mg were determined in venous blood collected from the occipital sinus. Blood PO(2) declined significantly with dive length; however, oxy-haemoglobin saturation remained greater than 30% for all R. leukops sampled. No changes were observed in blood PCO(2), pH, [HCO(3)(-)], or plasma glucose, with increasing dive length. Despite repeated dives lasting more than 2 h, plasma lactate remained less than 3 mmol l(-1) for all R. leukops sampled, indicating the absence of anaerobiosis. Compensatory acid-base adjustments associated with anaerobiosis (e.g. declining [Cl(-)], increasing total [Ca] and [Mg]) were likewise absent, with plasma-ion concentrations remaining stable with increasing dive length. Results indicate that R. leukops utilises aquatic respiration to remain aerobic during prolonged dives, thus effectively avoiding the development of a metabolic and respiratory acidosis.