Circulating vasotocin in the snake Bothrops jararaca

1. There is biochemical and pharmacological evidence to suggest the presence of vasotocin in the blood and plasma of the snake Bothrops jararaca (Bj). 2. XE-64 extracts from Bj blood showed antidiuretic and hypotensive activities in rats and a contractile effect on rat isolated uterus, which was tot...

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Bibliographic Details
Published in:	Comparative biochemistry and physiology. Comparative physiology Vol. 103; no. 1; p. 59
Main Authors:	Silveira, P F, Schiripa, L N, Carmona, E, Picarelli, Z P
Format:	Journal Article
Language:	English
Published:	England 01-09-1992
Subjects:	Animals Biological Assay Blood Pressure - drug effects Chromatography, High Pressure Liquid Chromatography, Liquid Diuresis - drug effects Female Rats Snakes - blood Uterine Contraction - drug effects Vasotocin - blood Vasotocin - pharmacology
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Summary:	1. There is biochemical and pharmacological evidence to suggest the presence of vasotocin in the blood and plasma of the snake Bothrops jararaca (Bj). 2. XE-64 extracts from Bj blood showed antidiuretic and hypotensive activities in rats and a contractile effect on rat isolated uterus, which was totally dialysable and inhibited by thioglycollate. 3. Extracts from Bj whole plasma presented an antidiuretic activity which was only partially dialysable. 4. The plasma extracts also showed oxytocic properties. 5. When EDTA and Sep-Pak C18 extraction were used, a better recovery and characterization of vasotocin by HPLC was obtained. 6. These results indicate the occurrence of free and bound circulating vasotocin in Bj, in an equilibrium dependent of its enzymatic hydrolysis.
DOI:	10.1016/0300-9629(92)90241-H