Preparation and some properties of human serum kallikrein immobilized on ARM-Sepharose

A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. KM values for N-benzoyl-L-arginine ethyl ester and N-tosyl-L-arginine methyl ester hydrolysis of this preparation were 1.10 x 10(-3) M and 3.6 x 10(-4) M, respectively; pH optimum of hydrolysis of these esters wer...

Full description

Saved in:

Bibliographic Details
Published in:	Clinica chimica acta Vol. 93; no. 3; p. 329
Main Authors:	Kawalec, M, Kawiak, J, Yarovaya, G A, Dotsenko, V L, Orekhovich, V N
Format:	Journal Article
Language:	English
Published:	Netherlands 02-05-1979
Subjects:	Arginine - analogs & derivatives Arginine - metabolism Enzymes, Immobilized Humans Hydrogen-Ion Concentration Kallikreins - isolation & purification Kallikreins - metabolism Tosylarginine Methyl Ester - metabolism
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. KM values for N-benzoyl-L-arginine ethyl ester and N-tosyl-L-arginine methyl ester hydrolysis of this preparation were 1.10 x 10(-3) M and 3.6 x 10(-4) M, respectively; pH optimum of hydrolysis of these esters were found to be 8.2 and 8.5, respectively. The immobilized kallikrein possessed kininogenase activity and was capable of activating prekallikrein.
ISSN:	0009-8981
DOI:	10.1016/0009-8981(79)90282-1