Development and use of detection methods specific for Cucumber vein yellowing virus (CVYV)

• Published in: European journal of plant pathology Vol. 110; no. 8; pp. 811 - 821
• Main Authors: Martinez-Garcia, B, Marco, C.F, Goytia, E, Lopez-Abella, D, Serra, M.T, Aranda, M.A, Lopez-Moya, J.J
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-10-2004; Springer Nature B.V
• Subjects: amino acid sequences; Biological and medical sciences; coat proteins; Cucumber vein yellowing virus; cucumbers; Cucumis sativus; disease detection; Flowers & plants; Fundamental and applied biological sciences. Psychology; gene expression; host plants; Ipomovirus; molecular sequence data; Nucleic acids; nucleotide sequences; Phytopathology. Animal pests. Plant and forest protection; Plant extracts; plant viruses; Plant viruses and viroids; RNA probes; Spain; Plant pathology; emerging pathogens; Microbiology; Plant pathogen; Cucurbitaceae; Serology; Cucumis sativus; Method; hybridization; Virus; Plant; Dicotyledones; Ipomovirus; Emerging disease; Angiospermae; Spermatophyta; Diagnosis; Detection; Potyviridae
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-004-2491-7

Two methods for the detection of Cucumber vein yellowing virus (CVYV) on infected plants were developed, based on the information provided by cDNA clones covering the 3'-end of the genome of a Spanish isolate (CVYV-AILM). The sequenced portion of the CVYV-AILM genome showed a 96.6% aminoacid identity with that of a reported sequence of another CVYV isolate from Israel (Lecoq et al., 2000). The first detection method used a RNA specific probe for hybridization with nucleic acids extracted from infected plants. The probe was complementary to a portion of the CVYV genome including the C-terminal part of the NIb and most of the coat protein (CP) coding regions. The second detection method employed polyclonal antisera raised against recombinant viral CP expressed in bacteria. The specific antibodies were used to detect the presence of virus particles in plant extracts. Both procedures resulted in a highly specific detection of CVYV in plants infected with different isolates of the virus. No interference was observed with other cucurbit-infecting viruses. Sensitivities achieved were sufficient for routine diagnosis of the presence of the virus in plants.