Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging

• Published in: Advanced biosystems Vol. 2; no. 8
• Main Authors: Okumura, Hiroki, Nanizawa, Eri, Nakanishi, Anna, Yukawa, Hiroshi, Hashita, Tadahiro, Iwao, Takahiro, Baba, Yoshinobu, Ishikawa, Tetsuya, Matsunaga, Tamihide
• Format: Journal Article
• Language: English
• Published: 01-08-2018
• Subjects: 3D culture; fluorescence imaging; hepatocyte transplantation; quantum dots; spheroids
• ISSN: 2366-7478; 2366-7478
• DOI: 10.1002/adbi.201800137

Liver transplantation is the most effective treatment for patients with end‐stage liver diseases, but hepatocyte transplantation using spheroids is expected to become an alternative strategy owing to the limited number of liver donors. However, the effects of the transplantation route and spheroid size on cell dynamics and the organ‐specific accumulation of hepatocytes are not well characterized. In this study, the influence of three delivery routes (tail vein, portal vein, and spleen) and three spheroid sizes (50, 100, and 150 µm in diameter) on the accumulation of human fetal hepatocytes labeled with quantum dots in the liver and other organs (heart, lung, kidney, and spleen) of recipient mice is investigated. Ex vivo fluorescence imaging reveals that the accumulation of transplanted cells in the liver is highest for transplantation via the portal vein compared with other routes. The accumulation efficiency depends on spheroid size, and spheroids with a diameter of 50 µm display greater accumulation than that of spheroids of other sizes. It is concluded that hepatocyte transplantation via the portal vein using small spheroids of 50 µm in diameter may be effective for clinical applications.
Development of more effective hepatocyte transplantation method is essential for treatment for end‐stage liver diseases. Here, the influence of three delivery routes and three spheroid sizes on the accumulation of hepatocytes labeled with quantum dots in the liver and other organs are presented.