An N-terminal Ca2+-binding motif regulates the secretory pathway Ca2+/Mn2+-transport ATPase SPCA1

• Published in: The Journal of biological chemistry Vol. 294; no. 19; pp. 7878 - 7891
• Main Authors: Chen, Jialin, Smaardijk, Susanne, Mattelaer, Charles-Alexandre, Pamula, Filip, Vandecaetsbeek, Ilse, Vanoevelen, Jo, Wuytack, Frank, Lescrinier, Eveline, Eggermont, Jan, Vangheluwe, Peter
• Format: Journal Article
• Language: English
• Published: 11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A Elsevier Inc 10-05-2019; American Society for Biochemistry and Molecular Biology
• Subjects: breast cancer; calcium ATPase; calcium transport; Golgi; Hailey–Hailey disease; ion homeostasis; manganese; Membrane Biology; membrane transporter reconstitution; protein purification; proteoliposomes; membrane transporter reconstitution; calcium ATPase; ion homeostasis; protein purification; proteoliposomes; breast cancer; Golgi; manganese; Hailey–Hailey disease; calcium transport
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.RA118.006250

The Ca2+/Mn2+ transport ATPases 1a and 2 (SPCA1a/2) are closely related to the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) and are implicated in breast cancer and Hailey–Hailey skin disease. Here, we purified the human SPCA1a/2 isoforms from a yeast recombinant expression system and compared their biochemical properties after reconstitution. We observed that the purified SPCA1a displays a lower Ca2+ affinity and slightly lower Mn2+ affinity than SPCA2. Remarkably, the turnover rates of SPCA1a in the presence of Mn2+ and SPCA2 incubated with Ca2+ and Mn2+ were comparable, whereas the turnover rate of SPCA1a in Ca2+ was 2-fold higher. Moreover, we noted an unusual biphasic activation curve for the SPCA1a ATPase and autophosphorylation activity, not observed with SPCA2. We also found that the biphasic pattern and low apparent ion affinity of SPCA1a critically depends on ATP concentration. We further show that the specific properties of SPCA1a at least partially depend on an N-terminal EF-hand–like motif, which is present only in the SPCA1a isoform and absent in SPCA2. This motif binds Ca2+, and its mutation lowered the Ca2+ turnover rate relative to that of Mn2+, increased substrate affinity, and reduced the level of biphasic activation of SPCA1a. A biochemical analysis indicated that Ca2+ binding to the N-terminal EF-hand–like motif promotes the activity of SPCA1a by facilitating autophosphorylation. We propose that this regulation may be physiologically relevant in cells with a high Ca2+ load, such as mammary gland cells during lactation, or in cells with a low ATP content, such as keratinocytes.