Altered cyclooxygenase-1 and enhanced thromboxane receptor activities underlie attenuated endothelial dilatory capacity of omental arteries in obesity

• Published in: Life sciences (1973) Vol. 239; p. 117039
• Main Authors: Raees, Asmaa, Bakhamis, Aysha, Mohamed-Ali, Vidya, Bashah, Moataz, Al-Jaber, Mashael, Abraham, David, Clapp, Lucie H., Orie, Nelson N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15-12-2019; Elsevier BV
• Subjects: Acetylcholine; Adipose tissue; Adipose Tissue - blood supply; Adipose Tissue - metabolism; Adult; Apamin - pharmacology; Arginine; Arteries; Arteries - drug effects; Celecoxib; Celecoxib - pharmacology; Charybdotoxin; Charybdotoxin - pharmacology; COX-2 inhibitors; Cyclooxygenase 1 - metabolism; Cyclooxygenase 1 - physiology; Cyclooxygenase Inhibitors - pharmacology; Cyclooxygenase-1; Cyclooxygenase-2; Endothelial Cells - metabolism; Endothelial Cells - physiology; Endothelial dilatory capacity; Endothelium; Endothelium, Vascular - drug effects; Female; Gastroepiploic Artery - drug effects; Gastroepiploic Artery - metabolism; Gastrointestinal surgery; Humans; Indomethacin; Indomethacin - pharmacology; Isoforms; Male; Middle Aged; mRNA; Muscle Relaxation - drug effects; NG-Nitroarginine methyl ester; NG-Nitroarginine Methyl Ester - pharmacology; Nitric oxide; Nitric-oxide synthase; Obesity; Obesity, Morbid - metabolism; Omental adipose tissue arteries; Omentum - blood supply; Omentum - metabolism; Receptors, Thromboxane - metabolism; Receptors, Thromboxane - physiology; Risk analysis; Risk factors; Surgery; Thromboxane A2; Thromboxane A2 TP receptor activity; Vasodilation - drug effects; Obesity; Cyclooxygenase-1; Thromboxane A2 TP receptor activity; Omental adipose tissue arteries; Endothelial dilatory capacity; Thromboxane A TP receptor activity
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2019.117039

Obesity is a risk factor for endothelial dysfunction, the severity of which is likely to vary depending on extent and impact of adiposity on the vasculature. This study investigates the roles of cyclooxygenase isoforms and thromboxane receptor activities in the differential endothelial dilatory capacities of arteries derived from omental and subcutaneous adipose tissues in obesity.
Small arteries were isolated from omental and subcutaneous adipose tissues obtained from consented morbidly obese patients (n = 65, BMI 45 ± 6 kg m−2 [Mean ± SD]) undergoing bariatric surgery. Relaxation to acetylcholine was studied by wire myography in the absence or presence of indomethacin (10 μM, cyclooxygenase inhibitor), FR122047 (1 μM, cyclooxygenase-1 inhibitor), Celecoxib (4 μM, cyclooxygenase-2 inhibitor), Nω-Nitro-L-arginine methyl ester (L-NAME, 100 μM, nitric oxide synthase inhibitor) or combination of apamin (0.5 μM) and charybdotoxin (0.1 μM) that together inhibit endothelium-derived hyperpolarizing factor (EDHF). Contractions to U46619 (thromboxane A2 mimetic) were also studied.
Acetylcholine relaxation was significantly attenuated in omental compared with subcutaneous arteries from same patients (p < 0.01). Indomethacin (p < 0.01) and FR122047 (p < 0.001) but not Celecoxib significantly improved the omental arteriolar relaxation. Cyclooxygenase-1 mRNA and U46619 contractions were both increased in omental compared with subcutaneous arteries (p < 0.05). L-NAME comparably inhibited acetylcholine relaxation in both arteries, while apamin+charybdotoxin were less effective in omental compared with subcutaneous arteries.
The results show that the depot-specific reduction in endothelial dilatory capacity of omental compared with subcutaneous arteries in obesity is in large part due to altered cyclooxygenase-1 and enhanced thromboxane receptor activities, which cause EDHF deficiency.
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•Endothelial function was compared between omental and subcutaneous arteries derived from obese individuals.•Omental arteries suffered reduced endothelial dilatory capacity compared with subcutaneous arteries from same obese individual.•The reduction in omental endothelial dilatory capacity was more about EDHF than NO deficiency.•The reduction in omental dilatory capacity was orchestrated by altered COX-1 and enhanced thromboxane TP receptor activities.