Resin cast impressions as a tool for microscopic observations of fungal epiphytes on leaves

Resin cast impressions as a tool for microscopic observations of fungal epiphytes on leaves

A simple method for fungal epiphyte microscopic observations and preservation is described. A two-part clear casting resin, cotton leaves and two species of fungi were used to validate this protocol. We obtained very detailed images of fungal structures using this approach in addition to retaining t...

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Bibliographic Details
Published in:Journal of microbiological methods Vol. 186; p. 106237
Main Authors: Valencia, Cesar U., Sword, Gregory A.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-07-2021
Subjects:
Adaxial
Conidiation
Fume hood
Fungal epiphytes
Hazardous
Phyllosphere
Resin cast impression
Fume hood
Adaxial
Conidiation
Phyllosphere
Fungal epiphytes
Resin cast impression
Hazardous
Online Access:Get full text
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Description
Summary:A simple method for fungal epiphyte microscopic observations and preservation is described. A two-part clear casting resin, cotton leaves and two species of fungi were used to validate this protocol. We obtained very detailed images of fungal structures using this approach in addition to retaining the impressions for future reference. •Resin cast impressions can be used to study plant-fungi interactions on leaves.•Impressions can be used to observe and preserve microscopic features.•The approach accurately preserves morphological leaf and fungal structures.•Preserved features include leaf stomata and epiphyte reproductive structures.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2021.106237