A simple method to determine hematoxylin ripening
A simple spectroscopic method is proposed to control the "ripening" of Delafield's hematoxylin solution during natural "ripening" or fast oxidation by different agents. The hematoxylin solution has ripened sufficiently for use with tissue sections staining if the absorption...
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| Published in: | Biotechnic & histochemistry Vol. 74; no. 4; p. 181 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
1999
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| Abstract | A simple spectroscopic method is proposed to control the "ripening" of Delafield's hematoxylin solution during natural "ripening" or fast oxidation by different agents. The hematoxylin solution has ripened sufficiently for use with tissue sections staining if the absorption is 1.2-1.3 at 560 nm. The hematoxylin solution must first be diluted 15-fold using a 5% ammonium alum solution and the absorption must be measured in 0.5 cm cuvettes. |
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| AbstractList | A simple spectroscopic method is proposed to control the "ripening" of Delafield's hematoxylin solution during natural "ripening" or fast oxidation by different agents. The hematoxylin solution has ripened sufficiently for use with tissue sections staining if the absorption is 1.2-1.3 at 560 nm. The hematoxylin solution must first be diluted 15-fold using a 5% ammonium alum solution and the absorption must be measured in 0.5 cm cuvettes. |
| Author | Rudneva, O A Budantsev, A Y |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10555858$$D View this record in MEDLINE/PubMed |
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| Snippet | A simple spectroscopic method is proposed to control the "ripening" of Delafield's hematoxylin solution during natural "ripening" or fast oxidation by... |
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| StartPage | 181 |
| SubjectTerms | Animals Hematoxylin - analysis Hematoxylin - chemistry Liver - chemistry Liver - pathology Male Microscopy, Video - methods Rats Rats, Wistar Solutions Spectrum Analysis - methods |
| Title | A simple method to determine hematoxylin ripening |
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