A simple method to determine hematoxylin ripening

A simple method to determine hematoxylin ripening

A simple spectroscopic method is proposed to control the "ripening" of Delafield's hematoxylin solution during natural "ripening" or fast oxidation by different agents. The hematoxylin solution has ripened sufficiently for use with tissue sections staining if the absorption...

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Bibliographic Details
Published in:Biotechnic & histochemistry Vol. 74; no. 4; p. 181
Main Authors: Budantsev, A Y, Rudneva, O A
Format: Journal Article
Language:English
Published: England 1999
Subjects:
Animals
Hematoxylin - analysis
Hematoxylin - chemistry
Liver - chemistry
Liver - pathology
Male
Microscopy, Video - methods
Rats
Rats, Wistar
Solutions
Spectrum Analysis - methods
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Description
Summary:A simple spectroscopic method is proposed to control the "ripening" of Delafield's hematoxylin solution during natural "ripening" or fast oxidation by different agents. The hematoxylin solution has ripened sufficiently for use with tissue sections staining if the absorption is 1.2-1.3 at 560 nm. The hematoxylin solution must first be diluted 15-fold using a 5% ammonium alum solution and the absorption must be measured in 0.5 cm cuvettes.
ISSN:1052-0295
DOI:10.3109/10520299909047972