High-speed chemical imaging of dynamic and histological samples with stimulated Raman micro-spectroscopy

We report a shot noise limited high-speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over 200 cm in 12 µs at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters, the designed low noise imagin...

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Bibliographic Details
Published in:	Optics express Vol. 28; no. 10; pp. 15505 - 15514
Main Authors:	Audier, Xavier, Forget, Nicolas, Rigneault, Hervé
Format:	Journal Article
Language:	English
Published:	United States Optical Society of America - OSA Publishing 11-05-2020
Subjects:	Colonic Neoplasms - chemistry Diagnostic Imaging Engineering Sciences Humans Mannitol - chemistry Olive Oil - chemistry Optics Photonic Polymethyl Methacrylate - chemistry Polystyrenes - chemistry Sensitivity and Specificity Serum Albumin, Bovine - chemistry Signal-To-Noise Ratio Spectrum Analysis, Raman - methods Triazines
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Summary:	We report a shot noise limited high-speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over 200 cm in 12 µs at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters, the designed low noise imaging platform performs chemical imaging of dynamical processes such as Mannitol crystal hydration and reaches a signal to noise ratio sufficient to perform label free histological imaging on frozen human colon tissue slides.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1094-4087 1094-4087
DOI:	10.1364/OE.390850