Anti-MRSA Activity of Actinomycin X2 and Collismycin A Produced by Streptomyces globisporus WA5-2-37 From the Intestinal Tract of American Cockroach (Periplaneta americana)

• Published in: Frontiers in microbiology Vol. 11; p. 555
• Main Authors: Chen, Zhiyu, Ou, Peiyu, Liu, Lingyan, Jin, Xiaobao
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 07-04-2020
• Subjects: actinomycin X2; collismycin A; methicillin-resistant Staphylococcus aureus; Microbiology; Periplaneta americana; Streptomyces globisporus
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2020.00555

Methicillin-resistant
Staphylococcus aureus
(MRSA) is recognized as one of the serious pathogen that causes acquired infections worldwide. Its emerging need to discover novel, safe and potent anti-MRSA drugs. In this study, primary screening by anti-MRSA activity assay found one strain WA5-2-37 isolated from the intestinal tract of
Periplaneta americana
, exhibited great activity against MRSA ATCC 43300. The strain WA5-2-37 produced actinomycin X
2
and collismycin A which showed strong inhibition of MRSA with minimum inhibitory concentration (MIC) values of 0.25 and 8 μg/mL. The structures of the pure compounds were elucidated by analysis of mass spectrometry (MS),
1
H and
13
C nuclear magnetic resonance (NMR). The strain WA5-2-37 was considered as
Streptomyces globisporus
on the basis of morphological characteristics, genotypic data, and phylogenetic analysis. This is the first reported naturally occurring strain of
S. globisporus
isolated from the intestinal tract of
P. americana
, whereas it has almost been found from plants, marine, and soil previously. Moreover,
S. globisporus
has not been reported to produce any anti-MRSA substances previously, such as actinomycin X
2
and collismycin A. In conclusion, the insect-derived strain of
S. globisporus
WA5-2-37 was considered of great potential as a new strain of producing actinomycin X
2
, collismycin A or other anti-MRSA compounds.