Renal phenotype of young and old telomerase-deficient mice

• Published in: Mechanisms of ageing and development Vol. 150; pp. 65 - 73
• Main Authors: Schildhorn, Carolin, Jacobi, Christoph, Weißbrodt, Andrea, Hermstedt, Christine, Westhoff, Jens Hendrik, Hömme, Meike, Bhayadia, Raj, Gretz, Norbert, Falk, Christine Susanne, Schmitt, Roland, Bröcker, Verena, Kränzlin, Bettina, Melk, Anette
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01-09-2015
• Subjects: Aging - genetics; Aging - metabolism; Animals; Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis; Cyclin-Dependent Kinase Inhibitor p16 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Gene Expression Regulation; Humans; Kidney; Kidney - metabolism; Kidney - pathology; Knockout-mouse; Mice; Mice, Knockout; Regeneration; RNA; Senescence; Telomerase; Telomerase - deficiency; Telomere; Senescence; PAS; Terc; Knockout-mouse; VEGF; MT; G-CSF; TA; Telomere; Kidney; IFN; GFR; TNF-α; PDGF; TFI; FISH; IF; STASIS; Telomerase; HPF; SIPS
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/j.mad.2015.08.004

•The hallmark of human renal ageing phenotype is its impaired ability to regenerate.•Young late generation Terc−/− mice show mild functional and histological changes.•Senescence explains limited regenerative capacity of young late generation Terc−/− kidneys.•Late generation Terc−/− mice are an attractive model to investigate impaired renal regeneration.
Telomere shortening in the kidney explains the impaired regenerative capacity, but may not drive the ageing phenotype itself. We investigated kidneys from young and old Terc+/+ and Terc−/− mice of early (G1) and late (G4, G5) generations.
Functional parameters declined and age-related morphological changes increased in late generation Terc−/− mice and with further age. Podocyte loss was only seen in old G4 Terc−/−. Whereas p21CIP1/WAF1 was highest in old G1 and G4 Terc−/−, telomere shortening and p16INK4a expression, also significantly associated with later generation young Terc−/−, were not further induced in old Terc−/− mice. Both, young and old late generation Terc−/−, showed increased pro-inflammatory cytokine levels.
Young late generation Terc−/− animals show mild functional and histological abnormalities, the presence of cellular senescence explains their kidneys’ limited regenerative capacity. While these aspects resemble the situation seen in aged human kidneys, the lack of telomere shortening and p16INK4a induction in older Terc−/− animals differs from observations in old human kidneys and may result from clearance of senescent cells. This animal model is well suited to investigate the mechanisms of impaired renal regeneration in aged human kidney, but may not fully explain the natural course of the human renal ageing phenotype.