Bioindustrial applications of thermostable Endoglucanase purified from Trichoderma viride towards the conversion of agrowastes to value-added products

• Published in: Protein expression and purification Vol. 211; p. 106324
• Main Authors: Chaudhary, Nidhee, Grover, Monendra
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-11-2023
• Subjects: Biocatalysis; Citric acid; N-terminal amino acid sequence; SCP; Thermostability; Thermostability; SCP; Biocatalysis; N-terminal amino acid sequence; Citric acid
• ISSN: 1046-5928; 1096-0279
• DOI: 10.1016/j.pep.2023.106324

Importance of biocatalytic reactions and biotransformations mediated by fungal enzymes has increased tremendously in various industries. Endoglucanase obtained from Trichoderma viride has been utilized for bioconversion of agrowastes; wheat straw (WS) and corn stover (CS) as biomass into citric acid and single cell protein (SCP) as value-added products. The enzyme was purified to apparent homogeneity with Mr:44.67 kDa; purification-fold, yield, specific activity to be 19.5-, 29.2%, and 150.4 Units.mg−1, respectively, with thermostability up to 70 °C. The enzyme showed a novel N-terminal peptide and its computational analysis revealed a conserved ‘SG’ amino acid sequence alike microbial cellulases. The experimental results have shown the potential of endoglucanase for the conversion of agrowastes; wheat straw (WS) and corn stover (CS) into citric acid, maximum yield (KgM−3) found in submerged (WS:50;CS:45) fermentation process. Single-cell protein (SCP) production in WS (68 KgM−3) hydrolysate was superior to both CS hydrolysate (60 KgM−3) and YEPD (standard medium) (58 KgM−3).
•Bioconversion of agrowastes into citric acid and SCP utilizing novel endoglucanase.•Endoglucanase purified from T. viride has novel N-terminal aa sequence.•Industrially significant novel Endoglucanase was thermostable upto 70 °C.•Maximum yield (KgM−3) of CA obtained in submerged (WS:50; CS:45) fermentation.•SCP production (KgM−3) obtained in WSH: CSH: YEPD being 68:60:58, respectively.