Metarhizium indicum, a new species of entomopathogenic fungus infecting leafhopper, Busoniomimus manjunathi from India

• Published in: Journal of invertebrate pathology Vol. 198; p. 107919
• Main Authors: Senthil Kumar, C.M., Jacob, T.K., Devasahayam, S., Rajeshkumar, Kunhiraman C., Lad, Sneha S., D'Silva, Sharon, Geethu, C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-06-2023
• Subjects: Animals; Biocontrol; Epizootics; Garcinia; Hemiptera; Humans; India; Insecta - microbiology; Metarhizium - genetics; Multi-gene analyses; Phylogeny; Taxonomy; India; Garcinia; Multi-gene analyses; Taxonomy; Epizootics; Hemiptera; Biocontrol
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2023.107919

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•A new species of Metarhizium pathogenic to leafhopper infesting Garcinia is reported.•The fungus infecting the leafhopper was distinctly different from its related species.•Morphological features and multi-gene analyses confirmed the fungus as a new species.•The fungus was named Metarhizium indicum indicating its Indian origin.
A new species of entomopathogenic fungus, Metarhizium indicum, which derives its species epithet after its Indian origin is reported here. The fungus was found to cause natural epizootics in leafhopper (Busoniomimus manjunathi) infesting Garcinia gummi-gutta (Malabar tamarind), an evergreen spice tree native to South and Southeast Asia, known for its use as a culinary flavourant, dietary supplement and traditional remedy for various human ailments. The fungus was found to cause more than 60% mortality in field collected insects. The identity of the new species was established based on its distinct morphological characteristics and multi-gene sequence data analyses. Phylogenetic analyses using internal transcribed spacer region (ITS), DNA lyase (APN2) and a concatenated set of four marker genes [translation elongation factor 1-alpha (TEF), β-tubulin (BTUB), RNA polymerase II largest subunit (RPB1) and RNA polymerase II second largest subunit (RPB2)] along with marked differences in nucleotide composition and genetic distance unambiguously support our claim that the present fungus infecting Garcinia leafhopper is a new addition to the genus Metarhizium.