Improving Cold Atmospheric Pressure Plasma Efficacy on Breast Cancer Cells Control-Ability and Mortality Using Vitamin C and Static Magnetic Field

• Published in: Plasma chemistry and plasma processing Vol. 40; no. 2; pp. 511 - 526
• Main Authors: Mehrabifard, R., Mehdian, H., Hajisharifi, K., Amini, E.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-03-2020; Springer Nature B.V
• Subjects: Antioxidants; Ascorbic acid; Breast cancer; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Classical Mechanics; Inorganic Chemistry; Investigations; Magnetic fields; Mechanical Engineering; Original Paper; Survival; Vitamin C; Wound healing; Cancer cell; Cell viability; Cold plasma; Static magnetic field; Vitamin C
• ISSN: 0272-4324; 1572-8986
• DOI: 10.1007/s11090-019-10050-5

In the last decades, there have been numerous reports about the separate interactions of a magnetic field and cold atmospheric plasma (CAP) with the biological systems. We have investigated the combined effect of CAP with the static magnetic field (SMF) as an effective method for cancer cells treatment.
MDA
-
MB
-
231
breast cancer cells were cultured and treated with CAP with different input power and exposure times in the presence and absence of the SMF. Vitamin C was also used in medium, and cell viability was investigated in the presence and absence of this antioxidant compound. The MTT assay was employed to measure cell survival, and a
T
-test or one-way
ANOVA
was used to assess the significance level of quantitative data. In order to determine the migration rate of cancer cells, wound healing assay was carried out. Results show that the presence of the SMF and vitamin C as well as increasing the input power significantly decrease the survival and migration rate of the cells. The results of the present investigation will greatly contribute to improve the CAP efficiency in cancer therapy by using the SMF and vitamin C as a complement to conventional CAP therapies.