Bioanalytical method for the quantification of sunitinib and its n-desethyl metabolite SU12662 in human plasma by ultra performance liquid chromatography/tandem triple-quadrupole mass spectrometry

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 51; no. 4; pp. 934 - 941
• Main Authors: de Bruijn, Peter, Sleijfer, Stefan, Lam, Mei-Ho, Mathijssen, Ron H.J., Wiemer, Erik A.C., Loos, Walter J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11-03-2010; Elsevier
• Subjects: Administration, Oral; Adult; Analysis; Analytical, structural and metabolic biochemistry; Angiogenesis Inhibitors - administration & dosage; Angiogenesis Inhibitors - blood; Angiogenesis Inhibitors - pharmacokinetics; Biological and medical sciences; Biotransformation; Calibration; Chromatography, Liquid - standards; Dealkylation; Female; Fundamental and applied biological sciences. Psychology; General pharmacology; Human plasma; Humans; Indoles - administration & dosage; Indoles - blood; Indoles - pharmacokinetics; Light sensitivity; Medical sciences; Pharmacology. Drug treatments; Pyrroles - administration & dosage; Pyrroles - blood; Pyrroles - pharmacokinetics; Reference Standards; Reproducibility of Results; SU12662; Sunitinib; Tandem Mass Spectrometry - standards; UPLC–MS/MS; Sunitinib; Human plasma; SU12662; UPLC–MS/MS; Light sensitivity; Antineoplastic agent; Human; Ultra performance liquid chromatography; Biological fluid; Tandem mass spectrometry; Enzyme; Tyrosine kinase inhibitor; Metabolite; Transferases; Enzyme inhibitor; Quadrupole spectrometer; Blood plasma; Sensitivity; UPLC-MS/MS; Multikinase inhibitor; Antiangiogenic agent; Protein-tyrosine kinase; Mass spectrometry; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2009.10.020

A rapid and sensitive ultra performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS) method has been developed and validated for the quantitative determination of sunitinib and its
n-desethyl metabolite SU12662, in 100
μl aliquots of human potassium EDTA plasma with deuterated sunitinib as internal standard. As sunitinib was found to be extremely sensitive to light causing rapid conversion of the Z (
cis)-isomer to the E (
trans)-isomer, the sample extraction and cleaning-up were performed under sodium-light and in amber vials. The extraction involved a simple liquid–liquid extraction with
tert-butyl methyl ether. Chromatographic separations were achieved on an Aquity UPLC
® BEH C
18 1.7
μm, 2.1
mm
×
50
mm column eluted at a flow rate of 0.250
ml/min on a gradient of acetonitrile. The overall cycle time of the method was 4
min, with elution times of 1.05, 1.43, 0.95, and 1.34
min, for the E (
trans)- and Z (
cis)-isomers of sunitinib and the E (
trans)- and Z (
cis)-isomers of SU12662, respectively. The multiple reaction monitoring transitions were set at 399
>
326 (
m/
z), at 371
>
283 (
m/
z) and at 409
>
326 (
m/
z) for sunitinib, SU12662 and the internal standard, respectively. The calibration curves were linear over the range of 0.200 to 50.0
ng/ml with the lower limit of quantitation validated at 0.200
ng/ml for both sunitinib and SU12662. The within-run and between-run precisions were within 11.7%, while the accuracy ranged from 90.5 to 106.8%.