Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate

When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell.

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) Vol. 48; no. 39; p. 4740
Main Authors:	Ohtsuka, Keiichi, Sato, Shinobu, Sato, Yusuke, Sota, Kojiro, Ohzawa, Shinsuke, Matsuda, Tomoki, Takemoto, Kiwamu, Takamune, Nobutoki, Juskowiak, Bernard, Nagai, Takeharu, Takenaka, Shigeori
Format:	Journal Article
Language:	English
Published:	England 01-01-2012
Subjects:	Aptamers, Nucleotide - chemistry Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry HeLa Cells Humans Ions Peptides - chemistry Potassium - analysis Potassium - chemistry Streptavidin - chemistry
Online Access:	Get more information
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Description
Summary:	When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell.
ISSN:	1364-548X
DOI:	10.1039/c2cc30536d