Identification and characterization of Aspergillus species of fruit rot fungi using microscopy, FT-IR, Raman and UV–Vis spectroscopy

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 246; p. 119010
• Main Authors: Saif, F.A., Yaseen, S.A., Alameen, A.S., Mane, S.B., Undre, P.B.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05-02-2021
• Subjects: Aspergillus; Fruit; Fruit rot fungi; FTIR–ATR spectroscopy; Fungi; Microscopy; Morphology; Raman spectroscopy; Species; Spectroscopy, Fourier Transform Infrared; UV–Vis spectroscopy; Aspergillus; Fruit rot fungi; Morphology; FTIR–ATR spectroscopy; Raman spectroscopy; UV–Vis spectroscopy; Species
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2020.119010

During the investigation of fungal isolation from fruit, the major genera were Aspergillus, Penicillium, cladosporium, Alternaria, fusarium, Colletotrichum were found. Among them Aspergillus (15 species) was found major dominant on different fruits. Fifteen different Aspergillus species viz. Aspergillus brasiliensis, Aspergillus phoenicis, Aspergillus carbonarius, four Aspergillus flavus, Aspergillus acidus, two Aspergillus awamori, Aspergillus aculeatus, Aspergillus eucalypticola, Aspergillus oryzae and two Aspergillus Spp. have been differentiate and identify using morphology (microscopic technique), Fourier Transforms Infrared spectroscopy (FTIR), Raman Spectroscopy (RS) and UV–visible spectrophotometry (UV–vis). The fungal mass in powder form was used in present study. In FTIR the finger print region is important for the characterization of Aspergillus because this region is unique and contains peaks indicating the presence of DNA. From the results were found Fourier transform infrared (FTIR) technique and Raman spectroscopy a useful tool, sensitive, fast, economical, accurate, not require sample preparation and successfully used to identify fungi.
[Display omitted]
•Aspergillus species have been cultured, isolated, differentiate and identify using morphology, FTIR, Raman and UV-vis spectrophotometry.•The identical species have similar FTIR, Raman and UV‐Vis spectra, whereas it is different for different species.•Used methods are complementary in the presentation of biochemical components information about the types of Aspergillus and their classification.•These methods are reliable methods for rapid and accurate identification and discrimination between different species.