Light promotes the synthesis of lignin through the production of H2O2 mediated by diamine oxidases in soybean hypocotyls

• Published in: Journal of plant physiology Vol. 162; no. 12; pp. 1297 - 1303
• Main Authors: Su, G, An, Z, Liu, Y
• Format: Journal Article
• Language: English
• Published: Jena Elsevier 01-12-2005
• Subjects: Agronomy. Soil science and plant productions; amine oxidase (copper-containing); Amine Oxidase (Copper-Containing) - antagonists & inhibitors; Amine Oxidase (Copper-Containing) - metabolism; Biological and medical sciences; biosynthesis; Cell Wall - metabolism; cell walls; enzyme activity; enzyme inhibition; Fundamental and applied biological sciences. Psychology; Genetic engineering applications; Genetics and breeding of economic plants; Glycine max; Glycine max - metabolism; Guanidines - pharmacology; Hydrazines - pharmacology; hydrogen peroxide; Hydrogen Peroxide - metabolism; Hypocotyl - metabolism; hypocotyls; Light; lighting; lignin; Lignin - biosynthesis; oxidation; peroxidase; Peroxidase - metabolism; plant biochemistry; Plant breeding: fundamental aspects and methodology; plant ecology; plant physiology; polyamines; seedling growth; seedlings; signal transduction; soybeans; Lignin; Polyamine; Aminoguanidine; Hydrogen peroxide; 2-hydroxyethylhydrazine; Enzyme; Growth; Soybean hypocotyls; Glycine max; Amine oxidase (copper-containing); Cell wall; Hypocotyl; Leguminosae; Peroxidases; Dicotyledones; Diamine oxidases; Angiospermae; Spermatophyta; Peroxidase; Oxidoreductases
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2005.04.033

In order to analyze the relationship between polyamine oxidative degradation induced by light and the lignin synthesis in cell walls, the activities of diamine oxidases and peroxidase, the contents of H2O2 and lignin, and the growth of hypocotyls in soybean Glycine max (Linn.) Merr. grown under light or in darkness were investigated. In comparison with the dark treatment, light irradiation significantly inhibited the growth of soybean hypocotyls and promoted the activities of diamine oxidases and peroxidase as well as the accumulation of H2O2 and lignin. Treatments with the different concentrations of diamine oxidase inhibitors (2-hydroxyethylhydrazine and aminoguanidine) under the light condition inhibited diamine oxidase activity, and decreased the contents of H2O2 and lignin. The results provide evidence for the hypothesis that light irradiation could promote the accumulation of H2O2 and lignin in cell walls by activating polyamine oxidative degradation mediated by diamine oxidases.