Preparation and biological evaluation of radiolabeled-folate embedded superparamagnetic nanoparticles in wild-type rats

• Published in: Journal of radioanalytical and nuclear chemistry Vol. 287; no. 1; pp. 119 - 127
• Main Authors: Jalilian, Amir Reza, Hosseini-Salekdeh, Seyyedeh Leila, Mahmoudi, Morteza, Yousefnia, Hassan, Majdabadi, A., Pouladian, Majid
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2011
• Subjects: Bioavailability; Cations; Chemistry; Chemistry and Materials Science; Diagnostic Radiology; Hadrons; Heavy Ions; Inorganic Chemistry; Iron oxides; Nanomaterials; Nanoparticles; Nanostructure; Nuclear Chemistry; Nuclear Physics; Physical Chemistry; Rats; Tracers; Ga; SPION; Biodistribution; Folate; Quality control
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-010-0661-y

In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe
3
O
4
) was characterized by FT-IR. The particle size was shown to be ≈5–10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([
67
Ga]-SPION-folate) were prepared using
67
Ga with a high labeling efficiency (over 96%, RTLC method) and they also showed an excellent stability at room temperature for at least 2 days and were evaluated for their biodistribution in normal rats up to 24 h compared with free Ga
3+
cation and [
67
Ga]-SPION biodistribution. The biodistribution of the tracer among 3 other folate tracers were compared, showing lower liver uptake and higher blood circulation after 24 h leading to better bioavailability. The bone:muscle, kidney:muscle, lung:muscle, stomach:muscle ratios were 9.3, 9.32, 7.6 and 5.83 respectively. The developed folate-containing nano-system can be an interesting folate receptor tracer, capable of better cell membrane permeability while possessing paramagnetic properties for thermotherapy.