Design of a pseudo stir bar sorptive extraction using graphenized pencil lead as the base of the molecularly imprinted polymer for extraction of nabumetone

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 238; p. 118427
• Main Authors: Afsharipour, Roya, Dadfarnia, Shayessteh, Shabani, Ali Mohammad Haji, Kazemi, Elahe
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05-09-2020
• Subjects: Adsorption; Anti-Inflammatory Agents, Non-Steroidal - analysis; Anti-Inflammatory Agents, Non-Steroidal - blood; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - urine; Central composite design; Graphene oxide anchored pencil, lead; Graphite - chemistry; Humans; Metal Nanoparticles - chemistry; Molecular Imprinting - methods; Molecularly imprinted polymer; Molecularly Imprinted Polymers - chemistry; Nabumetone; Nabumetone - analysis; Nabumetone - blood; Nabumetone - isolation & purification; Nabumetone - urine; Silicate sol-gel stabilized Ag NPs; Silver - chemistry; Solid Phase Extraction - methods; Water - analysis; Nabumetone; Graphene oxide anchored pencil, lead; Central composite design; Silicate sol-gel stabilized Ag NPs; Molecularly imprinted polymer
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2020.118427

Molecularly imprinted polymer (MIP) was synthesized through the coprecipitation method on the graphene oxide anchored pencil lead as a substrate for the first time and applied as an efficient sorbent for pseudo stir bar sorptive extraction of nabumetone. The extracted analyte was
determined by a novel spectrophotometric method based on the aggregation of silicate sol-gel
stabilized silver nanoparticles in the presence of the analyte. The synthesized polymer was
characterized using Fourier transform infrared spectroscopy and field emission scanning electron
microscopy. Optimization of important parameters affecting the extraction efficiency was done
using central composite design whereas the spectrophotometric method was optimized via one at
a time variable. Under the optimal conditions, the calibration curve exhibited linearity in the concentration range of 1.5–20.0 μg L−1. A limit of detection of 0.20 μg L−1, an enhancement factor of 393 and relative standard deviations (at 10 μg L−1, n = 6) of 4.6% and 8.1% for intra- and inter-day analysis were obtained. The developed procedure was successfully utilized for the
quantification of traces of nabumetone in tap water and biological samples with the complex
matrix including human urine and serum.
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•The first synthesis of MIP for separation and preconcentration of nabumetone•The first chemical synthesis of MIP on GO anchored pencil lead as supporting material.•Application of the functionalized pencil lead in the pseudo stir bar sorptive extraction method.•The first application of silicate sol-gel (SSG) stabilized Ag NPs for determination of drug.•Develop. of a simple & sensitive method for determ. of NAB by a combination of MIP & LSPR.