Mutagenicity and recombinogenicity evaluation of bupropion hydrochloride and trazodone hydrochloride in somatic cells of Drosophila melanogaster

• Published in: Food and chemical toxicology Vol. 131; p. 110557
• Main Authors: Naves, Maria Paula Carvalho, de Morais, Cássio Resende, Spanó, Mário Antônio, de Rezende, Alexandre Azenha Alves
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-09-2019
• Subjects: Antidepressants; SMART; Somatic mutation and recombination test; Toxicity; SMART; Antidepressants; Toxicity; Somatic mutation and recombination test
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2019.06.004

The aim of the present study was to appraise the mutagenic and recombinogenic potential of bupropion hydrochloride (BHc) and trazodone hydrochloride (THc). We used standard (ST) and the high bioactivation (HB) crossings from Drosophila melanogaster in the Somatic Mutation and Recombination Test. We treated third-instar larvae from both crossings with different concentrations of BHc and THc (0.9375 to 7.5 mg/mL). BHc significantly increased the frequency of mutant spots in both crossings, except for the lowest concentration in the ST crossing. ST had also the mostly recombinogenic result, and in the HB, BHc was highly mutagenic. On the other hand, THc significantly increased the frequency of mutant spots in both the ST and HB crossings at all concentrations. The three initial concentrations were recombinogenic and the highest concentration was mutagenic for the THc. BHc and THc at high concentrations were toxic, even though their mutagenicity was not dose-related. THc significantly increased the frequency of mutant spots when metabolized, probably as a result of the production of 1-(3′-chlorophenyl) piperazine. BHc was essentially recombinogenic and when metabolized, it became mutagenic. THc was recombinogenic in both crossings. Further studies are needed to clarify the action mechanisms from BHc and THc.
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•The mutagenic and recombinogenic potential of antidepressants was studied in vivo.•In high concentrations BHc and THc were toxic to D. melanogaster.•BHc was recombinogenic at the ST cross and mutagenic at the HB cross.•THc was recombinogenic at the lowest concentrations and mutagenic at the highest.