Purification of the Protease Activator of Protein C of Human Blood Plasma Produced by the Micromycete Aspergillus ochraceus VKM F-4104D

• Published in: Applied biochemistry and microbiology Vol. 56; no. 1; pp. 32 - 36
• Main Authors: Komarevtsev, S. K., Popova, E. A., Kreyer, V. G., Miroshnikov, K. A., Osmolovskiy, A. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2020; Springer Nature B.V
• Subjects: Ammonium; Ammonium sulfate; Aspergillus ochraceus; Biochemistry; Biomedical and Life Sciences; Blood plasma; Fractionation; Hydrophobicity; Ion exchange; Life Sciences; Medical Microbiology; Microbiology; Protease; Protein C; Protein folding; Protein purification; Proteinase; Proteins; Purification; Venom; protein-C activator; micromycete proteases; chromatography
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683820010093

A method has been developed for the purification of a protease activator of protein C in human blood plasma from the culture fluid of micromycete
Aspergillus ochraceus
VKM F-4104D. The properties of this protein are similar to those of the protein-C activator from the venom of the snake
Agkistrodon contortrix contortrix
, which is used in modern laboratory diagnostics of protein C. The method combines fractionation by ammonium sulfate and hydrophobic, ion-exchange, and gel-permeation chromatography. As a result, a highly purified preparation of protein-C activator was obtained with a 350-fold increase in specific activity during the purification process.