A fuel-limited isothermal DNA machine for the sensitive detection of cellular deoxyribonucleoside triphosphates

A fuel-limited isothermal DNA machine has been built for the sensitive fluorescence detection of cellular deoxyribonucleoside triphosphates (dNTPs) at the fmol level, which greatly reduces the required sample cell number. Upon the input of the limiting target dNTP, the machine runs automatically at...

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) Vol. 52; no. 80; p. 11923
Main Authors:	Dong, Jiantong, Wu, Tongbo, Xiao, Yu, Xu, Lei, Fang, Simin, Zhao, Meiping
Format:	Journal Article
Language:	English
Published:	England 01-01-2016
Subjects:	A549 Cells Chromatography, High Pressure Liquid Deoxyribonucleosides - analysis Deoxyribonucleosides - chemistry Deoxyribonucleosides - metabolism DNA - chemistry Fluorescent Dyes - chemistry Humans Mass Spectrometry Nucleic Acid Amplification Techniques Spectrophotometry, Ultraviolet Temperature
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Summary:	A fuel-limited isothermal DNA machine has been built for the sensitive fluorescence detection of cellular deoxyribonucleoside triphosphates (dNTPs) at the fmol level, which greatly reduces the required sample cell number. Upon the input of the limiting target dNTP, the machine runs automatically at 37 °C without the need for higher temperature.
ISSN:	1364-548X
DOI:	10.1039/c6cc05988k