OsADK1, a novel kinase regulating arbuscular mycorrhizal symbiosis in rice

• Published in: The New phytologist Vol. 234; no. 1; pp. 256 - 268
• Main Authors: Guo, Rui, Wu, Ya‐Nan, Liu, Cheng‐Chen, Liu, Ying‐Na, Tian, Li, Cheng, Jian‐Fei, Pan, Zhiyong, Wang, Dong, Wang, Bin
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-04-2022
• Subjects: arbuscular mycorrhiza (AM); Arbuscular mycorrhizas; arbuscule development; Colonization; CRISPR; Extreme values; Fungi; Gene editing; Gene Expression Regulation, Plant; Inoculum; Kinases; Mutants; Mutation; Mycorrhizae - physiology; Oryza - metabolism; OsADK1; Phenotypes; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - metabolism; Rice; rice (Oryza sativa); Roots; Signal transduction; Symbionts; Symbiosis; Symbiosis - physiology; symbiotic programme; Transcription; Transcription factors; Transcriptomes; Transgenic plants; arbuscule development; arbuscular mycorrhiza (AM); rice (Oryza sativa); symbiotic programme; OsADK1
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.17979

Summary
Arbuscular mycorrhizal (AM) symbiosis relies on the formation of arbuscules for efficient nutrient exchange between plants and AM fungi. In this study, we identified a novel kinase gene in rice named OsADK1 (Arbuscule Development Kinase 1) that is required for arbuscule development.
By obtaining OsADK1pro::GUS transgenic rice plants and also generating Osadk1 mutants via CRISPR/Cas9 technique, OsADK1 was revealed to be specifically induced in the arbusculated cortical cells and mutations in OsADK1 resulted in an extremely low colonisation rate (c. 3%) of rice roots by AM fungus Rhizophagus irregularis.
In the mutant roots, the very few observed arbuscules nearly all arrested at an early ‘trunk‐forming’ phase without forming any branches. Increasing the inoculum strength of AM fungus or cocultivation with a wild‐type nurse plant did not result in the rescue of the arbuscule phenotype. Transcriptome sequencing of both nursed and un‐nursed Osadk1 mutants then revealed that the mutation of OsADK1 could greatly affect the AM symbiotic programme, including many key transcription factors such as RAM1 and WRI5.
OsADK1 therefore represents a new rice kinase that is required for arbuscule branching. Its identification opens a new window to explore the elaborate signal transduction pathway that determines arbuscule development during plant–fungus symbiosis.