Comparative genome analysis and characterization of the Salmonella Typhimurium strain CCRJ_26 isolated from swine carcasses using whole‐genome sequencing approach

• Published in: Letters in applied microbiology Vol. 66; no. 4; pp. 352 - 359
• Main Authors: Panzenhagen, P.H.N., Cabral, C.C., Suffys, P.N., Franco, R.M., Rodrigues, D.P., Conte‐Junior, C.A.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-04-2018
• Subjects: Antibiotic resistance; Antibiotics; Antimicrobial resistance; Carcasses; Epidemiology; Gene sequencing; Genes; Genomes; Livestock; Microbiology; multiple drug resistance; Pathogenicity; Pathogenicity islands; Pathogens; PFGE; Phenotypes; Plasmids; Pork; Salmonella; Salmonella pathogenicity islands; Swine; Virulence; Virulence factors; whole‐genome sequencing; Brazil; whole genome sequencing; PFGE; multiple drug resistance; Salmonella pathogenicity islands
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/lam.12859

Salmonella pathogenicity relies on virulence factors many of which are clustered within the Salmonella pathogenicity islands. Salmonella also harbours mobile genetic elements such as virulence plasmids, prophage‐like elements and antimicrobial resistance genes which can contribute to increase its pathogenicity. Here, we have genetically characterized a selected S. Typhimurium strain (CCRJ_26) from our previous study with Multiple Drugs Resistant profile and high‐frequency PFGE clonal profile which apparently persists in the pork production centre of Rio de Janeiro State, Brazil. By whole‐genome sequencing, we described the strain's genome virulent content and characterized the repertoire of bacterial plasmids, antibiotic resistance genes and prophage‐like elements. Here, we have shown evidence that strain CCRJ_26 genome possible represent a virulence‐associated phenotype which may be potentially virulent in human infection.
Significance and Impact of the Study
Whole‐genome sequencing technologies are still costly and remain underexplored for applied microbiology in Brazil. Hence, this genomic description of S. Typhimurium strain CCRJ_26 will provide help in future molecular epidemiological studies. The analysis described here reveals a quick and useful pipeline for bacterial virulence characterization using whole‐genome sequencing approach.
Significance and Impact of the Study: Whole‐genome sequencing technologies are still costly and remain underexplored for applied microbiology in Brazil. Hence, this genomic description of S. Typhimurium strain CCRJ_26 will provide help in future molecular epidemiological studies. The analysis described here reveals a quick and useful pipeline for bacterial virulence characterization using whole‐genome sequencing approach.