The susceptibility to autoxidation of erythrocytes in diabetic mice: Effects of melatonin and pentoxifylline

• Published in: Journal of biochemical and molecular toxicology Vol. 31; no. 12
• Main Authors: Şekeroğlu, Mehmet Ramazan, Huyut, Zübeyir, Çokluk, Erdem, Özbek, Hanefi, Alp, Hamit Hakan
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-12-2017
• Subjects: Alloxan; Animals; antioxidant enzymes; Antioxidants; Autoxidation; Complications; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Drug Evaluation, Preclinical; Erythrocytes; Erythrocytes - drug effects; Erythrocytes - metabolism; Free Radical Scavengers - pharmacology; Free Radical Scavengers - therapeutic use; Glutathione; Glutathione Peroxidase - blood; Malondialdehyde; Melatonin; Melatonin - pharmacology; Melatonin - therapeutic use; Mice; Oxidation; Oxidation-Reduction; Oxidative Stress; pentoxifylline; Pentoxifylline - pharmacology; Pentoxifylline - therapeutic use; Peroxidase; RBC; Rodents; pentoxifylline; RBC; antioxidant enzymes; melatonin; diabetes
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.21976

Oxidative stress had a great importance in development of complications in diabetes. We investigated effects of melatonin and pentoxifylline in diabetic mice. Swiss albino mice (n = 40) were divided into four groups: alloxan‐induced diabetes mellitus (DM), alloxan‐induced diabetes with melatonin supplementation (DM + MLT), alloxan‐induced diabetes with pentoxifylline supplementation (DM + PTX), and control. Glutathione‐peroxidase (GSH‐Px) activity, malondialdehyde (MDA) and reduced glutathione (GSH) levels, and susceptibility to oxidation of erythrocytes were measured. MDA levels were higher than control in the DM and DM + MLT. The DM had more MDA level than the DM + MLT and DM + PTX (P < 0.001). After in vitro oxidation, MDA levels of all groups were found higher than the control. However, they were significantly lower than the DM in DM + PTX and DM + MLT (P < 0.001). Although GSH levels of the DM and DM + PTX were less than the control, GSH‐Px activity of the DM was lower than the control and DM + PTX (P < 0.05). We suggest that there is increased oxidative stress and compromised antioxidant status of erythrocytes in diabetes; however, it can be effectively prevented by melatonin or pentoxifylline supplementation.