Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study

• Published in: Journal of cellular biochemistry Vol. 120; no. 10; pp. 17159 - 17166
• Main Authors: Eraslan, Ersen, Tanyeli, Ayhan, Polat, Elif, Yetim, Zeliha
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-10-2019
• Subjects: Animal tissues; Antioxidants; Apoptosis; Caspase; Caspase-3; Chemokines; Cytokines; Damage; evodiamine; Inflammation; Injuries; Interleukins; Ischemia; kidney ischemia reperfusion; Kidneys; nucler factor‐κB; Oxidative stress; Oxidizing agents; Reactive oxygen species; Renal failure; Reperfusion; kidney ischemia reperfusion; apoptosis; cytokines; evodiamine; nucler factor-κB; oxidative stress
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.28976

Renal ischemia/reperfusion (I/R) injury resulting in acute renal failure, is a major clinical problem due to its high mortality rate. Renal I/R increases the reactive oxygen species, secretion of inflammatory cytokines, chemokines and other factors. This suggests that initiating the apoptosis process in the presence of oxidative stress may play a role in life‐threatening conditions, such as ischemia. Ischemia reperfusion‐induced renal damage can result in renal failure and death. Although many treatment procedures have been carried out to reduce or destroy renal I/R damage in experimental models, so far, a routine method of treatment has not yet been found. For this reason, the current study was planned to investigate the possible protective effects of evodiamine on tissue damage caused by ischemia‐reperfusion in kidney tissue in rats and an experimental renal I/R model was used for this purpose. Four groups were formed in the study: the control, sham control, ischemia reperfusion (I/R), and evodiamine (10 mg/kg) + I/R groups. The effects of evodiamine against kidney I/R injury were investigated. TAS (total oxidant status), TOS (total oxidant status), interleukin‐1β (IL‐1β), IL‐6, IL‐10 and tumor necrosis factor‐α levels were determined by enzyme‐linked immunosorbent assay. The oxidative stress index was calculated from TAS and TOS levels. In addition, the renal ischemia reperfusion injury was examined histopathologically. The IL‐10 and TAS levels in the I/R group decreased when compared with the control and Sham groups, while these levels increased in the evodiamine group. Histopathologic examination revealed that caspase 3 and nuclear factor‐κB levels decreased in the evodiamine group compared with the I/R group. The application of evodiamine significantly reduced ischemia reperfusion‐induced kidney damage due to its antioxidant, anti‐inflammatory and antiapoptotic properties.
Evodiamine reduces the levels of pro‐inflammatory cytokines. Evodiamine downregulates apoptosis and nuclear factor‐κB (NF‐κB) expression.