IgG4‐positive plasma cells are more often detected in chronic periapical lesions arising from permanent rather than primary teeth

• Published in: International endodontic journal Vol. 54; no. 5; pp. 682 - 692
• Main Authors: Polanco, X. B. J., Bertasso, A. S., Silveira, H. A., Yamamoto de Almeida, L., Almeida, L. K. Y., Silva, R. A. B., Silva, L. A. B., Rossi, A., Nelson‐Filho, P., León, J. E.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-05-2021
• Subjects: Cysts; deciduous teeth; Dentistry; Dentition; Granuloma; Granulomas; Humans; IgG4; Immunoglobulin; Immunoglobulin A; Immunoglobulin D; Immunoglobulin G; Immunoglobulin M; Immunohistochemistry; Inflammation; Interferon regulatory factor 4; Lesions; Light chains; Periapical Granuloma; periapical lesion; permanent teeth; Plasma; Plasma Cells; Radicular Cyst; Root canals; Teeth; Tooth, Deciduous; Immunohistochemistry; IgG4; deciduous teeth; Immunoglobulin; permanent teeth; periapical lesion
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.13464

Aim
To characterize plasma cell subsets in chronic periapical lesions affecting permanent and primary teeth.
Methodology
Only chronic periapical lesions without root canal treatment were selected. Twenty‐one radicular cysts and 7 periapical granulomas affecting permanent teeth and 19 radicular cysts and 4 periapical granulomas affecting primary teeth were assessed for immunoglobulin (Ig) light chain (kappa and lambda), Ig heavy chain (IgG, IgG4, IgA, IgM and IgD) and plasma cell immunohistochemical markers (MUM1/IRF4, EMA and CD138). The data acquired were analysed by Student’s t test, Mann–Whitney U, Friedman test followed by Dunn's multiple comparison test and Spearman’s rank correlation.
Results
All cases were polyclonal (having similar kappa/lambda light chain ratios). IgG was most abundant compared to other Ig heavy chains (all, P < 0.001); like Ig light chains, but unlike IgA, there was greater expression of IgG in the primary compared to the permanent dentition, for both radicular cysts (P < 0.001) and periapical granulomas (P = 0.53). Notably, IgG4 expression was greater in the permanent than the primary dentition, for both radicular cyst (P < 0.05) and periapical granuloma (P = 0.65). IgM and IgD expression was scarce and variable, whereas plasma cell populations were detected efficiently through EMA, CD138 and MUM1/IRF4 markers, the latter being more sensitive in both dentitions.
Conclusions
There were slight variations in the Ig light and heavy chain profiles in chronic periapical lesions when comparing the permanent and primary dentitions. The ability of IgG4+ plasma cell infiltration to modulate inflammatory responses in chronic periapical lesions arising from permanent as opposed to primary teeth should be considered in future studies.