Induction of strong immune response against a multicomponent antigen of Mycobacterium tuberculosis in BALB/c mice using PLGA and DOTAP adjuvant

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica Vol. 126; no. 6; pp. 509 - 514
• Main Authors: Khademi, Farzad, Sahebkar, Amirhossein, Fasihi‐Ramandi, Mahdi, Taheri, Ramezan Ali
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01-06-2018
• Subjects: Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - pharmacology; Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antigens; Antigens, Bacterial - immunology; Bacillus Calmette-Guerin vaccine; Bacterial Proteins - immunology; BCG; Cytokines; DOTAP; Enzyme-linked immunosorbent assay; Escherichia coli - genetics; Evaporation; Fatty Acids, Monounsaturated - chemistry; Female; Formulations; Fused protein; HspX/EsxS; Immune response; immune responses; Immune system; Immunity, Cellular; Immunization; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; Interferon; Interferon-gamma - blood; Interferon-gamma - immunology; Interleukin-4 - blood; Interleukin-4 - immunology; Interleukins; Lactic Acid - chemistry; Mice; Mice, Inbred BALB C; Mycobacterium tuberculosis; Nanoparticles; Nanoparticles - chemistry; PLGA; Polyglycolic Acid - chemistry; Polylactide-co-glycolide; Proteins; Quaternary Ammonium Compounds - chemistry; Recombinant Fusion Proteins - immunology; Rodents; Splenocytes; Tuberculosis; Tuberculosis Vaccines - immunology; Vaccination; PLGA; DOTAP; Mycobacterium tuberculosis; HspX/EsxS; immune responses
• ISSN: 0903-4641; 1600-0463
• DOI: 10.1111/apm.12851

A promising strategy for preventing illness and death caused by Mycobacterium tuberculosis (Mtb) is vaccination. In this study, we aimed to evaluate the capacity of a multicomponent vaccine comprising HspX/EsxS‐fused protein, PLGA (poly (lactide‐co‐glycolide)) and DOTAP (1, 2‐dioleoyl‐3‐trimethylammonium propane) in eliciting immune responses against Mtb in BALB/c mice. A preparation of PLGA nanoparticles (NPs) containing fused protein and DOTAP adjuvant was made using double emulsion solvent evaporation (w/o/w) and lipid film hydration methods, respectively. After three subcutaneous immunization of BALB/c mice with various formulations, ELISA technique was used to measure interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) cytokines levels in splenocytes as well as serum anti‐HspX/EsxS IgG1 and IgG2a titers. The results of the current study showed that PLGA/HspX/EsxS/DOTAP formulation was able to induce higher levels of FN‐γ, IgG1, and IgG2a responses compared with BCG as the positive control, HspX/EsxS, HspX/EsxS/DOTAP and PLGA/HspX/EsxS formulations. Our results suggest that PLGA NPs, as delivery system, and DOTAP, as adjuvant, have a good potential to enhance immune responses against HspX/EsxS antigen after subcutaneous immunization of BALB/c mice.