Erroneous Measurement of Plasma Volume in the Rabbit by T-1824

• Published in: The American journal of physiology Vol. 194; no. 3; pp. 522 - 526
• Main Authors: Zizza, F, Reeve, E. B
• Format: Journal Article
• Language: English
• Published: United States 01-09-1958
• Subjects: Animals; Blood Volume Determination; Coloring Agents; Old Medline; Plasma Volume; Rabbits; BLOOD VOLUME/determination; DYES
• ISSN: 0002-9513; 2163-5773
• DOI: 10.1152/ajplegacy.1958.194.3.522

1 From the Department of Medicine, University of Colorado Medical Center, Denver, Colorado
When measurements of plasma volume are made in rabbits with T-1824 and rabbit albumin labeled with I 131 , the dye value averages 1.09 of the labeled albumin value. This dye value is in error, and the probable cause is loss of a fraction of the dye from the circulation immediately after the dye injection. Comparison of the later rates of loss of T-1824 and I 131 -albumin from the circulation shows that the dye is lost at nearly double the rate of loss of I 131 -albumin. T-1824 is thus an unsatisfactory label for rabbit albumin, and cannot be used for measurements of rabbit plasma volume. When F cells (body hematocrit/venous hematocrit) is measured with P 32 -labeled cells and I 131 -albumin the average value in rabbits is 0.895.