One-step melting curve analysis-based McSpoligotyping reveals genotypes of Mycobacterium tuberculosis in a coastal city, China

• Published in: Archives of microbiology Vol. 203; no. 7; pp. 4579 - 4585
• Main Authors: Pan, Jieru, Ye, Haimei, Wu, Zuda, Xiao, Jianbin, Liu, Jie
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-09-2021; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Ecology; Epidemiology; Genotypes; Life Sciences; Melting; Melting curve; Microbial Ecology; Microbiology; Mycobacterium tuberculosis; Oligonucleotides; Original Paper; Spoligotyping; Technology assessment; Tuberculosis; Beijing China; China; Spacer oligonucleotide typing; Molecular typing; McSpoligotyping
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02441-0

A one-step 3 h melting curve analysis-based technology for spacer oligonucleotide typing (McSpoligotyping) analyzed with the SITVIT2 database was applied to detect epidemiological events of
Mycobacterium tuberculosis
(MTB) in a coastal city of China from 2016 to 2018. 306 MTB isolates were identified by Fuzhou tuberculosis designated hospitals between 2016 and 2018. The results showed that the MTB isolates were divided into Beijing family and non-Beijing families, accounting for 45.42% (139/306) and 54.58% (167/306), respectively. H and T families were the prevalent genotypes in non-Beijing families. Herein, the spoligotyping technology has practical application for the classification and tracing of tuberculosis in the public.
Graphic abstract