Clinical significance of human neutrophil antigen-1 antibodies in children with neutropenia

• Published in: International journal of hematology Vol. 118; no. 5; pp. 627 - 635
• Main Authors: Goda, Satoshi, Karakawa, Shuhei, Okada, Satoshi, Kawaguchi, Hiroshi, Kurita, Emi, Noma, Mitsunori, Yamaoka, Aiko, Komatsu, Mayumi, Yanai, Ayaka, Kashihara, Mayu, Fujii, Teruhisa, Onodera, Rie, Taniguchi, Kikuyo, Aizawa, Mika, Kobayashi, Masao
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01-11-2023; Springer Nature B.V
• Subjects: Antibodies; Antigens; Children; Flow cytometry; Fluorescence; Hematology; Immunofluorescence; Leukocytes (granulocytic); Leukocytes (neutrophilic); Medicine; Medicine & Public Health; Neutropenia; Neutrophils; Oncology; Original Article; Human neutrophil antigen; Autoimmune neutropenia; Autoantibody; Childhood
• ISSN: 0925-5710; 1865-3774
• DOI: 10.1007/s12185-023-03661-4

Primary autoimmune neutropenia in young children is characterized by chronic neutropenia and positivity for antibodies against human neutrophil antigens (HNAs). This study analyzed the clinical characteristics of 402 children with neutropenia to identify differences between those with and without HNA-1 antibodies (HNA1abs). HNAabs in sera were detected by granulocyte immunofluorescence testing using flow cytometry. Relative fluorescence intensity (RFI) values were used to divide patients into positive (PG,
n
 = 302), borderline (BG,
n
 = 34), and negative (NG,
n
 = 66) groups. The antibodies reacted to HNA-1a alone (59%), HNA-1b alone (1%), and HNA-1a/1b (40%). The PG had a significantly lower absolute neutrophil count before definitive diagnosis and a 1.6- to 2-times greater risk of hospitalization during neutropenia than the other groups. The median duration of neutropenia was longest in the PG at 25 months, followed by 20 months in the BG and 14 months in the NG. This large-scale cohort characterizes clinically distinct groups using the RFI value for HNA1abs in young children with neutropenia. Detection of HNA1abs may aid in understanding the clinical characteristics of children with neutropenia.