Eco-friendly Mycogenic Synthesis of ZnO and CuO Nanoparticles for In Vitro Antibacterial, Antibiofilm, and Antifungal Applications

• Published in: Biological trace element research Vol. 199; no. 7; pp. 2788 - 2799
• Main Authors: Mohamed, Asem A., Abu-Elghait, Mohammed, Ahmed, Nehad E., Salem, Salem S.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-07-2021; Springer Nature B.V
• Subjects: Antibacterial activity; Antibiotics; Antifungal activity; Antifungal agents; Bacteria; Biochemistry; Biogenesis; Biomedical and Life Sciences; Biotechnology; Copper oxides; Disease resistance; Extracellular; Extracellular enzymes; Fungi; Fungicides; Gram-negative bacteria; Heavy metals; Life Sciences; Medical equipment; Metals; Microbiological strains; Microorganisms; Minimum inhibitory concentration; Nanoparticles; Nutrition; Oncology; Pathogens; Proteins; Qualitative analysis; Synthesis; Zinc; Zinc oxide; Zinc oxides; Anti-biofilm; Mycosynthesis; Antifungal; Antibacterial; Zinc oxide nanoparticles; Copper oxide nanoparticles
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-020-02369-4

Mycogenic synthesis of medically applied zinc oxide (ZnO) and copper oxide (CuO) nanoparticles (NPs) were exploited using
Penicillium chrysogenum
. The biogenesis and capping processes of the produced nano-metals were conducted by functional fungal extracellular enzymes and proteins. The obtained ZnO-NPs and CuO-NPs were characterized. Also, the antibacterial activity and minimum inhibitory concentration (MIC) values of ZnO-NPs and CuO-NPs were determined. Also, antibiofilm and antifungal activities were investigated. Results have demonstrated the ability of the bio-secreted proteins to cape and reduce ZnO and CuO to hexagonal and spherical ZnO-NPs and CuO-NPs with particle size at 9.0–35.0 nm and 10.5–59.7 nm, respectively. Both ZnO-NPs and CuO-NPs showed high antimicrobial activities not only against Gram-positive and Gram-negative bacteria but also against some phytopathogenic fungal strains. Besides this, those NPs showed varied antibiofilm effects against different microorganisms. Quantitative and qualitative analyses indicated that CuO-NPs had an effective antibiofilm activity against
Staphylococcus aureus
and therefore can be applied in diverse medical devices. Thus, the mycogenic green synthesized ZnO-NPs and CuO-NPs have the potential as smart nano-materials to be used in the medical field to limit the spread of some pathogenic microbes.