Transcriptome Analysis During Tetrasporogenesis of Gracilariopsis lemaneiformis and Preliminary Study of the Expressions of Its Meiotic Genes

• Published in: Journal of Ocean University of China Vol. 22; no. 2; pp. 541 - 554
• Main Authors: Zhu, Weifang, Yang, Ze, Xu, Di, Yang, Jinxin, Zhang, Yingyue, Pan, Xiaohui
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01-04-2023; Springer Nature B.V; The Key Laboratory of Marine Genetics and Breeding,Ministry of Education,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China
• Subjects: Earth and Environmental Science; Earth Sciences; Encyclopaedias; Encyclopedias; Endoplasmic reticulum; Fertility; Genes; Genomes; Gracilariopsis lemaneiformis; Meiosis; Meteorology; MLH1 protein; MRE11 protein; MSH2 protein; MSH6 protein; Oceanography; Photosynthesis; Ribosomes; RNA transport; Spores; Sporogenesis; Transcriptomes; tetrasporogenesis; transcriptome; meiosis related genes; Gracilariopsis lemaneiformis
• ISSN: 1672-5182; 1993-5021; 1672-5174
• DOI: 10.1007/s11802-023-5289-y

The transcriptomes of three different parts of the fertile tetrasporophyte of
Gracilariopsis lemaneiformis
, including tip (T), middle (M), and subjacent (S) parts, with a gradual tetrasporangium maturity were analyzed and compared to identify the genes involved in the process of tetrasporogenesis. The number of differentially expressed genes (DEGs) for the Gple-S
versus
Gple-T comparison was 10296, and the numbers of DEGs for the Gple-S
versus
Gple-M and Gple-T
versus
Gple-M comparisons were 7435 and 1337, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, and the results showed the enrichment of 132 KEGG pathways (corrected
P
<0.05). A total of 58 DEGs related to meiosis were screened and blasted against 18 meiosis-related genes (
dmc1, mlh1, mnd1, msh4, msh2, msh6, mre11, pds5, pms1, rad21, rad50, rad51, smc1, smc2, smc4, smc5, smc6
, and
spo11
), including four meiosis-specific genes. The transcriptome comparison indicated that in the T part, the meiosis, ribosome, and RNA transport-related genes were mostly up-regulated compared with those in the other two groups. In the M part, the genes related to ribosomes and the endoplasmic reticulum were also up-regulated compared with those in the lower part. Finally, in the S part, the genes associated with photosynthesis were mostly up-regulated, which might be helpful to the recovery from spore formation and release.