Mice heterozygous for the Serpinb6a null mutation show deficits in central auditory function after acoustic trauma

• Published in: Neuroreport Vol. 32; no. 16; pp. 1287 - 1292
• Main Authors: Tan, Justin, Kaiserman, Dion, O’Leary, Stephen J, Bird, Phillip I.
• Format: Journal Article
• Language: English
• Published: England Wolters Kluwer Health, Inc. All rights reserved 02-11-2021
• Subjects: Animals; Auditory Threshold - physiology; Evoked Potentials, Auditory, Brain Stem - physiology; Hearing Loss, Noise-Induced - genetics; Hearing Loss, Noise-Induced - metabolism; Loss of Function Mutation; Mice; Mice, Knockout; Serpins - genetics; Serpins - metabolism
• ISSN: 0959-4965; 1473-558X
• DOI: 10.1097/WNR.0000000000001727

Complete deficiency of the serine protease inhibitor gene, SERPINB6, is responsible for autosomal-recessive, nonsyndromic sensorineural hearing loss in humans. A mouse model of this deafness gene identifies Serpinb6a expression in the neurosensory epithelium and fibrocytes of the cochlea. Homozygous Serpinb6a mutant mice display an exaggerated hearing loss after exposure to moderate acoustic trauma. It is unknown if and how heterozygous Serpinb6a mice show increased vulnerability to acoustic trauma.
We exposed Serpinb6a+/- and Serpinb6a+/+ mice to acoustic trauma and measured their hearing function prior to, 3 and 14 days postexposure, analysing shifts in hearing threshold and amplitudes of Wave I and II of the auditory brainstem-evoked response (ABR) to 4, 8, 16 and 32 kHz tones.
Shifts in hearing threshold and Wave I amplitude of Serpinb6a+/- mice were not significantly different from Serpinb6a+/+ mice at both time points and all frequencies tested (P > 0.05, Mann-Whitney test). However, Wave II amplitudes at 16 and 32 kHz tones, were more severely diminished in Serpinb6a+/- mice (P < 0.05). To exclude any effects of ageing on auditory function in Serpinb6a+/- mice, hearing function of unexposed Serpinb6a+/- mice was measured at start and end of the experimental period. The shift in Wave II amplitude of exposed Serpinb6a+/- mice was significantly lower than unexposed Serpinb6a+/- mice only at 16 and 32 kHz (P < 0.01), confirming acoustic trauma as the main cause of hearing deficits in Serpinb6a+/- mice.
These results suggest that heterozygous Serpinb6a humans may be vulnerable to noise.