Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance

• Published in: Journal of microbiology and biotechnology Vol. 33; no. 6; pp. 797 - 805
• Main Authors: Cong, Pham Van, Anh, Hoang Le Tuan, Vinh, Le Ba, Han, Yoo Kyong, Trung, Nguyen Quang, Minh, Bui Quang, Duc, Ngo Viet, Ngoc, Tran Minh, Hien, Nguyen Thi Thu, Manh, Hoang Duc, Lien, Le Thi, Lee, Ki Yong
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28-06-2023
• Subjects: alpha-Glucosidases; Molecular Docking Simulation; Molecular Structure; Research article; Saponins - chemistry; Saponins - pharmacology; Vernonia - chemistry; stigmastane-type steroidal glycoside; α-glucosidase inhibitor; Vernonia gratiosa; vernogratiosides A–C
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2212.12040

Species belonging to the
(Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemical components. A phytochemical investigation resulted in the separation of three new stigmastane-type steroidal saponins (1 - 3), designated as vernogratiosides A-C, from whole plants of
. Their structures were elucidated based on infrared spectroscopy (IR), one-dimensional (1D) and two-dimensional nuclear magnetic resonance (2D NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and electronic circular dichroism analyses (ECD), as well as chemical reactivity. Molecular docking analysis of representative saponins with α-glucosidase inhibitory activity was performed. Additionally, the intended substances were tested for their ability to inhibit α-glucosidase activity in a laboratory setting. The results suggested that stigmastane-type steroidal saponins from
are promising candidate antidiabetic agents.