Association between adipose tissue expression and serum levels of leptin and adiponectin in women with polycystic ovary syndrome

• Published in: Genetics and molecular research Vol. 12; no. 4; pp. 4292 - 4296
• Main Authors: Lecke, S B, Morsch, D M, Spritzer, P M
• Format: Journal Article
• Language: English
• Published: Brazil 28-02-2013
• Subjects: Adiponectin - blood; Case-Control Studies; Female; Humans; Leptin - blood; Obesity - blood; Polycystic Ovary Syndrome - blood; Subcutaneous Fat - metabolism
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2013.February.28.16

We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and adiponectin were determined by commercially available enzyme-linked immunosorbent assays. Adipose tissue total RNA was reserve-transcripted into complementary DNA samples, which were used as templates for quantitative real-time PCR amplification. Positive correlations were found between serum and mRNA levels for both leptin (r = 0.321; P = 0.005) and adiponectin (r = 0.266; P = 0.024). Determination of leptin and adiponectin serum levels could serve as an indirect method to assess adipocyte production, since leptin and adiponectin are predominantly produced by subcutaneous adipocytes in women.