Sciatic Nerve Plastic Surgery using Autologous Adipose Tissue

• Published in: Sovremennye tekhnologii v medit͡s︡ine Vol. 15; no. 1; pp. 30 - 36
• Main Authors: Velichanskaya, A G, Bugrova, М L, Pogadaeva, E V, Ermolina, E A, Yudintsev, A V, Ermolin, I L
• Format: Journal Article
• Language: English
• Published: Russia (Federation) Privolzhsky Research Medical University 01-01-2023
• Subjects: Adipose Tissue; Animals; Biotechnologies; Male; Rats; Rats, Wistar; Saline Solution; Sciatic Nerve - surgery; Silicon; Surgery, Plastic; sciatic nerve regeneration; autologous adipose tissue; sciatic nerve plastic surgery
• ISSN: 2076-4243; 2309-995X
• DOI: 10.17691/stm2023.15.1.04

is to study structural alterations of autologous omental adipose tissue in a silicon conduit and to evaluate its possible use for regeneration of the sciatic nerve in diastasis.
Mature outbred male Wistar rats have been used in the study. The animals were divided into 7 experimental groups with complete transection of the sciatic nerve on the right side at the mid-third level of the thigh. The ends of the transected nerve were pulled apart, inserted into a silicon conduit, and secured to the epineurium. The conduit of group 1 (control) was filled with a saline solution; in group 2, it was filled with an autologous omental adipose tissue with saline solution. Intravital labeling of the omental adipose tissue with the lipophilic PKH 26 dye (in group 3) was used for the first time to find out whether the omental cells were involved in formation of the regenerating nerve. Diastasis in groups 1-3 was 5 mm, the postoperative period was 14 weeks. The dynamics of the omental adipose tissue changes in groups 4-7 was assessed by placing the omental tissues into the conduit covering 2 mm of diastasis. The postoperative period was 4, 14, 21, and 42 weeks.
In group 2 (omental adipose tissue + saline), the clinical condition of the damaged limb after 14 weeks may be evaluated as satisfactory and approximating to the intact parameters as compared to group 1 where the conduit was filled with a saline solution only. The sum of large and medium-sized nerve fibers in group 2 was 2.7 times greater than that in group 2. The milled omental adipose tissue inside the conduit changed its volume and structure in nerve diastasis and was constantly utilized up to complete elimination over time. The omental cells integrated into the newly formed nerve in the graft area.
As a graft, the adipose tissue of the autologous omentum produces a stimulating effect on the post-traumatic regeneration of the sciatic nerve.