Possible effects of clinoptilolite on small intestinal ischemia-reperfusion injury caused by experimental mesenteric artery occlusion

• Published in: Türk biyokimya dergisi Vol. 47; no. 5; pp. 633 - 639
• Main Authors: Emre Bostanci, Meriç, Hepokur, Ceylan, Caner, Armağan, Can Mollaoğlu, Murat, Karadayi, Kürşat
• Format: Journal Article
• Language: English
• Published: De Gruyter 28-10-2022
• Subjects: clinoptilolite; ischemia-reperfusion; ischemic small intestines; RT-qPCR; TNF-α
• ISSN: 1303-829X; 1303-829X
• DOI: 10.1515/tjb-2021-0244

Abstract
Objectives
Mesenteric ischemia is a surgical emergency caused by poor blood supply to the intestines. In ischemia, the decrease in blood flow to the tissue causes acidosis and cell death through anaerobic metabolism. Clinoptilolite is one of the most abundant natural zeolites, and it is used for its ion exchange and adsorbent properties. Clinoptilolite has been reported to have an immune-enhancing, anti-carcinogenic, and antioxidant effect
in-vitro
/
in-vivo
studies. Clinoptilolite’s histological and biochemical effects on ischemic small intestines.
Methods
The experimental animals were randomly divided into sham, control, and clinoptilolite treatment group. Clinoptilolite was administered intraperitoneally after ischemia/reperfusion. Cardiac blood was stored for biochemical analysis. Total antioxidant levels and total oxidant levels were analyzed from the sera taken from groups. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expressions in blood samples were determined by RT-qPCR. At the end of the reperfusion, terminal ileum tissues were taken for histological tests.
Results
The mean TNF-α expression level was 3.89 in the control group and 2.91 in the clinoptilolite treatment group. The mean IL-6 expression levels were 2.32 in the control group and 1.49 in the clinoptilolite treatment group.
Conclusions
clinoptilolite administration provided healing in the rat ischemia-reperfusion injury model.