Characterization of the coccoid cyanobacterium Myxosarcina sp. KIOST-1 isolated from mangrove forest in Chuuk State, Federated States of Micronesia

• Published in: Ocean science journal Vol. 52; no. 3; pp. 359 - 366
• Main Authors: Kim, Ji Hyung, Lee, JunMo, Affan, Md-Abu, Lee, Dae-Won, Kang, Do-Hyung
• Format: Journal Article
• Language: English
• Published: Seoul Korea Institute of Ocean Science and Technology and the Korean Society of Oceanography 01-09-2017; Springer Nature B.V; 한국해양과학기술원
• Subjects: Aquatic Pollution; Biochemistry; Biodiversity; Carbohydrates; Earth and Environmental Science; Earth Sciences; Exploitation; Fatty acids; Lipids; Mangrove swamps; Mangroves; Marine & Freshwater Sciences; Myxosarcina; Oceanography; Phylogeny; Polyunsaturated fatty acids; Proteins; Symbionts; Waste Water Technology; Water Management; Water Pollution Control; 해양학; Chuuk; mangrove forest; sp. KIOST-1; fatty acids; multiple fissions; Federated States of Micronesia
• ISSN: 1738-5261; 2005-7172
• DOI: 10.1007/s12601-017-0029-0

Mangrove forests are known to be inhabited by diverse symbiotic cyanobacterial communities that are capable of N
2
fixation. To investigate its biodiversity, root sediments were collected from a mangrove forest in Chuuk State, Federated States of Micronesia (FSM), and an entangled yellow-brown coccoid cyanobacterium was isolated. The isolated cyanobacterium was reproduced by multiple fission and eventually produced baeocytes. Phylogenetic analysis revealed that the isolate was most similar to the genera
Myxosarcina
and
Chroococcidiopsis
in the order Pleurocapsales. Compositions of protein, lipid and carbohydrate in the cyanobacterial cells were estimated to be 19.4 ± 0.1%, 18.8 ± 0.4% and 31.5 ± 0.1%, respectively. Interestingly, total fatty acids in the isolate were mainly composed of saturated fatty acids and monounsaturated fatty acids, whereas polyunsaturated fatty acids were not detected. Based on the molecular and biochemical characteristics, the isolate was finally classified in the genus
Myxosarcina
, and designated as
Myxosarcina
sp. KIOST-1. These results will contribute to better understanding of cyanobacterial biodiversity in the mangrove forest in FSM as well as the genus
Myxosarcina
, and also will allow further exploitation of its biotechnological potential on the basis of its cellular characteristics.