Dataset of potential targets for Mycobacterium tuberculosis H37Rv through comparative genome analysis

• Published in: Bioinformation Vol. 4; no. 6; pp. 245 - 248
• Main Authors: Asif, Siddiqui M, Asad, Amir, Faizan, Ahmad, Anjali, Malik S, Arvind, Arya, Neelesh, Kapoor, Hirdesh, Kumar, Sanjay, Kumar
• Format: Journal Article
• Language: English
• Published: Singapore Biomedical Informatics Publishing Group 31-12-2009
• Subjects: Hypothesis; Mycobacterium tuberculosis; BLASTX; targets; Mycobacterium tuberculosis H37Rv; DEG
• ISSN: 0973-8894; 0973-2063; 0973-2063
• DOI: 10.6026/97320630004245

Mycobacterium tuberculosis is the causative agent of the disease, tuberculosis and H37Rv is the most studied clinical strain. We use comparative genome analysis of Mycobacterium tuberculosis H37Rv and human for the identification of potential targets dataset. We used DEG (Database of Essential Genes) to identify essential genes in the H37Rv strain. The analysis shows that 628 of the 3989 genes in Mycobacterium tuberculosis H37Rv were found to be essential of which 324 genes lack similarity to the human genome. Subsequently hypothetical proteins were removed through manual curation. This further resulted in a dataset of 135 proteins with essential function and no homology to human.