Genomic relatedness of Staphylococcus aureus phages of the International Typing Set and detection of serogroup A, B, and F prophages in lysogenic strains

• Published in: Canadian journal of microbiology Vol. 46; no. 11; p. 1066
• Main Authors: Doskar, J, Pallová, P, Pantůcek, R, Rosypal, S, Růzicková, V, Pantůcková, P, Kailerová, J, Klepárník, K, Malá, Z, Bocek, P
• Format: Journal Article
• Language: English
• Published: Canada 01-11-2000
• Subjects: Bacteriophage Typing; Cluster Analysis; Deoxyribonucleases, Type II Site-Specific; DNA Probes; DNA, Circular - genetics; DNA, Circular - isolation & purification; DNA, Viral - genetics; DNA, Viral - isolation & purification; Lysogeny; Proviruses - classification; Proviruses - genetics; Proviruses - isolation & purification; Restriction Mapping; Staphylococcus Phages - classification; Staphylococcus Phages - genetics; Staphylococcus Phages - isolation & purification
• ISSN: 0008-4166
• DOI: 10.1139/cjm-46-11-1066

On the basis of HindIII-restriction digest analysis of genomic DNAs, the S. aureus bacteriophages of the International Typing Set were divided into five clusters designated as A, F, Ba, Bb, and Bc. The clusters A and F include all the phages of serogroups A and F and correspond to species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb, and Bc that differ significantly each from the other in their restriction patterns. The clusters Ba and Bb may represent two separate species, while the cluster Bc may include more than one phage species. For each of the phage serogroups A, B, and F, common HindIII-restriction fragments of phage 3A (1700 bp), of 53 (4060 bp), and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B, and F. These probes were very effective, making it possible to detect up to three different prophages in a given lysogenic strain at the same time. Restriction enzyme maps of phages 3A, 53, and 77, each representing a different serogroup, were constructed. The restriction maps of phage 3A and that of phage 77 are linear, whereas that of phage 53 is circular and exhibits a circular permutation. DNAs of the phages of serogroups A and F have cohesive ends. On each restriction map, the sites corresponding to specific probes are indicated. The size of intact genomic DNA of all phages estimated by PFGE varies within the range of 41.5-46.2 kb.