Differentiation of Saccharomyces species by lipid and metabolome profiles from a single colony

• Published in: Food microbiology Vol. 103; p. 103964
• Main Authors: Ruiz-de-Villa, Candela, Poblet, Montse, Bordons, Albert, Reguant, Cristina, Rozès, Nicolas
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-05-2022
• Subjects: Cell Differentiation; Fatty acids; Fermentation; Lipids; Metabolome; S. kudriavzevii; S. uvarum; Saccharomyces; Saccharomyces cerevisiae; Squalene; Trehalose; Wine; Wine yeast; S. kudriavzevii; Trehalose; Squalene; Fatty acids; Saccharomyces cerevisiae; Wine yeast; S. uvarum
• ISSN: 0740-0020; 1095-9998
• DOI: 10.1016/j.fm.2021.103964

Yeast metabolism depends on growing conditions, which include the chemical composition of the medium, temperature and growth time. Historically, fatty acid profiles have been used to differentiate yeasts growing in liquid media. The present study determined the fatty acids of Saccharomyces species in colonies. Using the same method, the effect of that the number of colonies and growth time had on solid media allowed us to determine the metabolomic profiles of the cells. Our results showed that the lipid and metabolomic profiles of the cells evolved as the colony grew. Interestingly, some strains of Saccharomyces cerevisiae have been were differentiated using the fatty acid profile of a colony; concretely indeed EC1118 and QA23 strains were separated from ICV-K1 and BM4x4. The synthesis of saturated fatty acids was greater than that of unsaturated fatty acids during the first two days of cell growth on a solid medium compared to a liquid medium. Unsaturated fatty acids subsequently became predominant. Finally, this methodology could be useful for carrying out physiological studies in a complete or defined solid growth medium allowing the supplementation of compounds, which inhibit or activate the growth of yeasts.
•Lipid and metabolome profiles of Saccharomyces species growing on YPD agar were determined through GC-MS.•We differentiated the Saccharomyces species from the fatty acid profile using one colony. .•Higher ratio of SaturatedFA/UnsaturatedFA in yeast cells growing on a solid than in liquid medium after 2 days of growth.