Atomic force microscopy fishing of GP120 on immobilized aptamers and its mass spectrometry identification

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 8; no. 2; pp. 115 - 124
• Main Authors: Bukharina, N. S., Ivanov, Yu. D., Pleshakova, T. O., Frantsuzov, P. A., Andreeva, E. Yu, Kaysheva, A. L., Izotov, A. A., Pavlova, T. I., Ziborov, V. S., Radko, S. P., Archakov, A. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-04-2014; Springer Nature B.V
• Subjects: Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Ligands; Mass spectrometry; Medicinal Chemistry; Microscopy; Proteins; mass spectrometry; atomic force microscopy; aptamer; gp120 HIV-1; fishing
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750814020036

A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.