The synthesis of TNF-alpha conjugates with alendronic acid

• Published in: Russian journal of bioorganic chemistry Vol. 42; no. 6; pp. 638 - 645
• Main Authors: Volosnikova, E. A., Demin, I. F., Levagina, G. M., Lebedev, L. R., Zakabunin, A. I., Danilenko, E. D.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2016; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedical materials; Biomedicine; Bioorganic Chemistry; Conjugates; Conjugation; Crosslinking; Homogeneity; Hydroxyapatite; Life Sciences; Organic Chemistry; Solid phases; Stoichiometry; alendronic acid; binding to hydroxyapatite; conjugation; tumor necrosis factor alpha recombinant human
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162016060145

Conjugates of recombinant human tumor necrosis factor alpha (TNFα) and alendronic acid linked through the protein sulfhydryl, carboxyl, and amino groups were obtained with crosslinking agents of different types. The conjugation reactions were conducted in solution and on a solid phase. Unlike the conjugation reactions in solution, the method involving immobilization of active components on a hydroxyapatite column was shown to result in the conjugates with a specified stoichiometry and a high degree of homogeneity. The TNFα conjugates retained the specific cytolytic activity and demonstrated the higher affinity to hydroxyapatite, an analogue of the bone mineral matrix, than TNFα.