Impact of primary infection site of Fusarium species on head blight development in wheat ears evaluated by IR-thermography

• Published in: European journal of plant pathology Vol. 147; no. 4; pp. 855 - 868
• Main Authors: Al Masri, A., Hau, B., Dehne, H.-W., Mahlein, A.-K., Oerke, E.-C.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-04-2017; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Disease detection; Ecology; Experiments; Fungal infections; Fusarium graminearum; Life Sciences; Pesticides; Plant Pathology; Plant Sciences; Thermography; Triticum aestivum; Wheat; Wheat; Fusarium head blight; Thermography
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-016-1051-2

The effect of the primary infection site by
Fusarium graminearum
and
F. culmorum
within wheat ears on Fusarium head blight (FHB) was investigated under controlled conditions. FHB development was assessed visually and thermographically following inoculation by: (i) spraying ears, or injecting inoculum into spikelets on (ii) tip, (iii) centre and (iv) base of the ears, separately.
Fusarium
infection significantly increased the temperature span within ears 6 days post inoculation (dpi), especially infections starting at the ear tip. The temperature difference between air and ear was negatively correlated to FHB severity and enabled disease detection even 29 dpi.
F. culmorum
caused significant higher disease severity neither reflected in the frequency of infected kernels nor in thousand kernel weight (TKW). Spray inoculations had the strongest effect on TKW, whereas tip inoculations had no effect. Centre and base inoculations had intermediate effects on TKW, although FHB levels did not differ with the same trend among inoculation scenarios. The overall low correlations among FHB severity, infected kernels and TKW are explained by the pathogen spread within ears – downwards more than upwards – and the effect on yield formation which is lower for infections of the upper parts of ears. An exponential model showed high goodness of fit for gradients of infected kernels within ears (
R
2
 ≥ 70) except tip infection with
F. culmorum
. This study confirmed that FHB is a function of the primary infection site within ears. Thermography was useful to differentiate among infection scenarios and may be applied in breeding for FHB resistance.