Colonic healing after portal ischemia and reperfusion: an experimental study with oxidative stress biomarkers

Colonic healing after portal ischemia and reperfusion: an experimental study with oxidative stress biomarkers

This experimental study aimed to evaluate colon healing after portal ischemia followed by reperfusion. Seventy male Wistar rats randomly distributed in four groups were used: Group 1, colonic anastomosis (n = 20); Group 2, portal ischemia-reperfusion (n = 20); Group 3, colonic anastomosis and portal...

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Bibliographic Details
Published in:Redox report : communications in free radical research Vol. 12; no. 6; p. 267
Main Authors: Rolim, Marcia Farias, Riger, Cristiano J, Eleutherio, Elis C A, Colão, Cristiane da Fonseca, Pereira, Gerson Cotta, Schanaider, Alberto
Format: Journal Article
Language:English
Published: England 01-12-2007
Subjects:
Anastomosis, Surgical
Animals
Carbon - chemistry
Collagen - chemistry
Collagen - metabolism
Colon - pathology
Immunohistochemistry
Lipid Peroxidation
Lipids - chemistry
Male
Malondialdehyde - chemistry
Oxidative Stress
Oxygen - metabolism
Rats
Rats, Wistar
Reperfusion Injury
Thiobarbituric Acid Reactive Substances
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Summary:This experimental study aimed to evaluate colon healing after portal ischemia followed by reperfusion. Seventy male Wistar rats randomly distributed in four groups were used: Group 1, colonic anastomosis (n = 20); Group 2, portal ischemia-reperfusion (n = 20); Group 3, colonic anastomosis and portal ischemia-reperfusion (n = 20); and Group 4, control (n = 10). In the postoperative period, these rats were re-allocated into subgroups and lipid peroxidation and protein oxidation plasma levels were evaluated on days 1 and 5 by thiobarbituric acid reactive substances (TBARS) and slot-blotting assays, respectively. A segment of the right colon was also removed for collagen analysis. Both malondialdehyde (MDA) and protein carbonyl levels (oxidative markers of lipids and proteins) presented a significant increase after reperfusion in Group 3 on days 1 (P < 0.002) and 5 (P < 0.0001). In this same group, an extensive inflammatory process showing decreased fibroplasia was observed, with deficiency in collagen deposition on both sides of the anastomosis edges. Taken together, these results indicate that portal congestion followed by reperfusion induces an oxidative stress, which impaired the mechanism of colon anastomotic healing.
ISSN:1743-2928
DOI:10.1179/135100007X239261