Immunodetection of caspase-3 by Western blot using glutaraldehyde

Cell death by apoptosis triggers the engagement of a conserved intracellular machinery of execution, involving mainly the activation of the caspase family of cysteine proteases. Caspase-3 is a common effector of most of the apoptotic pathways and is able to cleave several target proteins whose degra...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 415; no. 2; pp. 203 - 205
Main Authors:	Nestal de Moraes, Gabriela, Carvalho, Érika, Maia, Raquel C., Sternberg, Cinthya
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 15-08-2011
Subjects:	Antibodies - immunology Antineoplastic Agents - pharmacology Blotting, Western - methods Caspase 3 - analysis Caspase 3 - immunology Caspase 3 - metabolism Cell Line, Tumor Glutaral - chemistry Humans Immunoassay - methods Recombinant Proteins - analysis Recombinant Proteins - immunology Recombinant Proteins - metabolism
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Summary:	Cell death by apoptosis triggers the engagement of a conserved intracellular machinery of execution, involving mainly the activation of the caspase family of cysteine proteases. Caspase-3 is a common effector of most of the apoptotic pathways and is able to cleave several target proteins whose degradation will contribute to the execution phase of the cell demise program. Here we present a modification of the Western blot protocol to improve sensitivity of caspase-3 detection, providing a valuable tool to access its activation in biological specimens.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2011.04.032